Figure 2 : The expression of NF-κB mRNA and asRNA in IL- 1β-treated hepatocytes.

mRNA (upper panel) and asRNA (lower panel) expression was analyzed via strand-specific RT-PCR in the absence (–) or presence (+) of IL-1β for 4 h. Total RNA from purified hepatocytes treated without or with IL-1β (1 nM) was analyzed as in Figure 1. The expression of both mRNAs and asRNAs transcribed from the genes encoding the NF-κB p65 and p50 subunits and IκB-α in the IL-1β signaling pathway was analyzed with specific PCR primer sets (Table 1). EF mRNA was used as an internal control for RT-PCR. RT(–) indicates a negative PCR control without RT to evaluate genomic DNA contamination.

Nishizawa et al.  2012 1:10DOI : 10.7243/2050-0874-1-10