Figure 4 : The effects of sense oligonucleotides on hepatocyte mRNA levels.

Sense oligonucleotides were designed according to the secondary structure predictions. Hepatocytes were transfected with each sense oligonucleotide in duplicate and incubated with IL-1β for 4 h. Total RNA from these cells was subjected to strand-specific RT and real-time PCR (n=3). The mRNA levels were normalized to those of EF mRNA. The relative levels of mRNA normalized to EF mRNA are shown as the mean ± SD (n=3) and expressed as a percentage of the mock transfection value (–). *P < 0.05. **P < 0.01.

Nishizawa et al.  2012 1:10DOI : 10.7243/2050-0874-1-10