Table 2. The expression levels of the mRNA and asRNA in IL-1b-treated hepatocytes.


Gene

asRNA level
[Sense primer]
(copies/mg RNA)1

mRNA level
[Antisense primer]
(copies/mg RNA)2

mRNA[AS]: asRNA
ratio3

mRNA level
[Oligo(dT) primer]
(copies/mg RNA)2

mRNA[dT]: asRNA
ratio3


IL-23A 4.38 × 105 7.71 × 105 1.8 1.01 × 106 2.3
CCL2 2.30 × 104 1.20 × 107 521.6 1.51 × 107 655.6
CCL20 8.24 × 104 5.05 × 106 61.3 1.10 × 107 133.9
CX3CL1 9.70 × 105 2.10 × 106 2.2 8.28 × 106 8.5
CD69 5.37 × 103 3.87 × 106 720.2 7.98 × 106 1484.6

1. A gene-specific sense primer was used for RT to synthesize cDNA to the asRNA. To determine the asRNA levels, real-time PCR was performed with the cDNA using known concentrations of gene-specific cDNA fragments as standards, as described in Materials and Methods. The expression level is shown as number of copies per mg of total RNA used for RT.

2. A gene-specific antisense primer (AS) or an oligo(dT) primer was used in RT to synthesize cDNA to the mRNA, and real-time PCR was performed to determine the copy numbers of the mRNA.

3. The mRNA:asRNA ratio was calculated by dividing the mRNA level by the asRNA level. IL-23A, interleukin 23, α subunit; CCL, chemokine C-C motif ligand; and CX3CL1, chemokine C-X3-C motif ligand 1.

Nishizawa et al.  2012 1:10DOI : 10.7243/2050-0874-1-10