Table 4. Sense oligonucleotides corresponding to mRNAs.


Name Position1 Sequence (5’->3’)2

IL-23, a subunit (IL-23A):
Se1 37– 56 AsAsTsCCATCAATGCAGACsAsTsC
Se2 219–238 GsAsAsGCTGGCAGACAGCTsGsCsA
Se3 433–452 TsCsCsTTCAGTTCTAACAGsAsAsC
Se4 567–586 AsAsCsAGTTTAGAGGATTGsTsTsA

CCL2:

   
Se1 22– 41 TsTsAsAGTAATGTTAAACTsTsAsT
Se2 108–127

TsCsCsATTTTTTTATTTCTsCsTsG


CCL20:    
Se1 69– 88 GsGsTsTTCACCTGCACATCsAsCsT
Se2 243–261 GsTsTsTAGCTATTTAATGTsTsAsA
Se3 243–261 TsAsAsTTTCCCACAATCTAsTsGsA

CX3CL1:    
Se1 1143–1162 AsCsTsTGTGCATGTGTGTAsCsTsT
Se2 1325–1344

AsCsAsAAGTGTCTACTGAAsGsCsA

Se3 1334–1353 CsTsAsCTGAAGCAGAGAGCsAsGsC

CD69 molecule:

   
Se1 8– 27 GsCsAsTAGAGGTATAGGTGsAsCsT
Se2 463–482

GsAsCsCAATGCTTATGAAAsAsCsA

Se3 731–750

GsTsGsGCAGATCTCTGTCAsGsGsA


NF-κB, p65 subunit:

   
Se1 256–275 GsAsAsCTCTTGAGACCCTGsCsTsT
Se2 346–365

TsTsAsTATCCAGTGTCTTCsCsTsC

Se3 396–415

GsCsAsACGCTCCTAGGAGCsAsGsC

Se4 486–505 AsAsCsTCTCCATGCTGAGCsAsGsT

1. The first nucleotide of the 3’-untranslated region of rat mRNA is designated as +1, and the position of each sense oligonucleotide is shown by nucleotide positions.
2. The small ‘s’ indicates a phosphorothioate bond. Conserved single-stranded regions (loops or bulges) in the secondary structure are underlined.

Nishizawa et al.  2012 1:10DOI : 10.7243/2050-0874-1-10