Figure 3 :  Rough eye phenotype of the sarcoglycan knockdown flies.

(A)GMR-GAL4/+;+;+ (B)GMR-GAL4/+;UAS-IR-dscgαi1/UAS-IR-dscgαi1;+ (C)GMR-GAL4/+;UAS-IR-dscgαi2/UAS-IR-dscgαi2;+ (D)GMR-GAL4/+;+;UASIR- dscgβi1/UAS-IR-dscgβi1 (E)GMR-GAL4/+;UAS-IR-dscgβi2/UAS-IR-dscgβi2;+ (F)GMR-GAL4/+;+;UAS-IR-dscgδi1/UAS-IR-dscgδi1 (G)GMR-GAL4/ UAS-IR-dscgδi2 (strain8441);+;UAS-IR-dscgδi2(strain8545)/+ (H)GMR-GAL4/+;UAS-IR-dscgαi2/+;+ (I)GMR-GAL4/+;UAS-IR-dscgβi2/+;+ (J)GMRGAL4/+;+; UAS-IR-dscgδi1/+ (K)GMR-GAL4/+;UAS-IR-dscgαi2/UAS-IR-dscgβi2;+ (L)GMR-GAL4/+;UAS-IR-dscgβi2/+;UAS-IR-dscgδi1/+ (M)GMRGAL4/+; UAS-IR-dscgαi2/+;UAS-IR-dscgδi1/+ (N) Reduction of dscgα, dscgβ or dscgδ mRNA levels in larval tissues. rp49 mRNA was used as an internal control. The graph shows the ratio of knockdown fly values to the wild type. Error bars show standard deviations (n=3). mRNAs were prepared from the knockdown flies in which the expression of each sarcoglycan double strand RNAs was induced by Act5C-GAL4. (Cont)Act5C-GAL4/+ (αi1)UAS-IRdscgαi1/+; Act5C-GAL4/+ (αi2)UAS-IR-dscgαi2/+;Act5C-GAL4/+ (βi1)UAS-IR-dscgβi1/Act5C-GAL4 (βi2)UAS-IR-dscgβi2/+;Act5C-GAL4/+ (δi1)UAS-IRdscgδi1/ Act5C-GAL4 (δi2)UAS-IR-dscgδi2/Act5C-GAL4 (O) Western blots of extracts from third-instar larvae of control (Cont), βi1 and βi2 knockdown flies were probed with anti-dScgβ and anti-αTubulin antibodies. (Cont)Act5C-GAL4/+ (βi1)UAS-IR-dscgβi1/Act5C-GAL4 (βi2)UAS-IR-dscgβi2/+;Act5CGAL4/+

Yamaguchi et al.HOAJ BIOLOGY  2012 1:7DOI : 10.7243/2050-0874-1-7