Table 1: Cloning into pETDuet vector.

All the used primer pairs, restriction enzymes and DNA resources of the generated constructs for in vitro binding assay using pETDuet vector are listed. F’ and R’ respectively represent forward and reverse primers. The sequences of the used restriction enzyme sites are underlined.

Yamaguchi et al.HOAJ BIOLOGY  2012 1:7DOI : 10.7243/2050-0874-1-7