Figure 11 : Representative sections cut from spleens isolated on day 12 post-immunization from control 

(A-G, top row) and EAE (H-N, bottom row) mice were labeled by immunohistochemistry with antibodies to STAT1 (A and H), STAT2 (B and I), STAT3 (C and J),
STAT4 (D and K), STAT5 (E and L) and STAT6 (F and M). In EAE mice, numerous secondary lymphatic follicles formed and were scattered across the spleen. The dark
brown immunostaining formed by STAT antibodies binding to each specific STAT protein was confined within the follicles, except STAT2 staining, which was localized in
other areas of the spleen, but not in the follicles. The numbers of positively stained cells for all splenic STAT proteins were higher in control mice than in EAE mice.
The intensity of the immunostaining for all splenic STAT proteins was also stronger in control mice than in EAE mice. G and N are negative controls in which the
primary antibody was replaced by normal serum. Magnification=40X.

Shindler et al.Immunology Innovation  2013 1:3DOI : 10.7243/2053-213X-1-3