Figure 2 : Protein was extracted from spleens isolated from EAE mice 12 days post-immunization.

(A) Western blot of protein extracts demonstrates expression of STAT family members in spleens of EAE (lanes 1–4, n=4)
and control mice (lanes 5–9, n=5). Expression was analyzed in both cytosolic (left column) and nuclear (right column)
fractions of protein extracts. β-actin expression was also quantified in each lane. (B) Densitometry analysis of Western
blots was used to quantify STAT protein expression, presented as a ratio of the density of the STAT protein band to the
β-actin band. There was a significant decrease (*P<0.05; **P<0.01) of STAT1, STAT3, STAT4, STAT5 and STAT6 protein
levels in both cytosolic (top row) and nuclear (bottom row) fractions of protein extracts from the spleens of EAE mice as
compared with spleens from control mice. In contrast, STAT2 expression did not change in either cytosolic or nuclear extracts
of spleen after EAE induction. Values are presented as mean ± SEM.

Shindler et al.Immunology Innovation  2013 1:3DOI : 10.7243/2053-213X-1-3