Figure 3 : Protein was extracted from thymuses isolated from EAE mice 12 days post-immunization.

The density of bands on Western blots of thymic protein extracts are shown as a ratio of the STAT1,
STAT2, STAT3, STAT4, STAT5 or STAT6 band density to the β-actin band density. Thymic protein
extracts from EAE (n=4) and control (n=5) mice were separated into cytosolic (left column) and
nuclear (right column) fractions. There was a significant decrease (*P<0.05) of STAT5 expression
in both cytosolic and nuclear fractions from EAE mice as compared with extracts from control mice.
There was a trend toward decreased expression of STAT6 protein in both cytosolic and nuclear fractions
from EAE mice, however this did not reach significance (P>0.05). STAT1, STAT2, STAT3 and STAT4 levels
were equivalent between EAE and control mice. Values are presented as mean ± SEM.

Shindler et al.Immunology Innovation  2013 1:3DOI : 10.7243/2053-213X-1-3