Figure 5 : Protein was extracted from thymuses isolated from EAE mice 5 days
post-immunization.

The density of bands on Western blots of thymic protein extracts are shown as a ratio of the
STAT1, STAT3, STAT4, STAT5 or STAT6 band density to the β-actin band density. Thymic protein
extracts from EAE (n=5) and control (n=6) mice were separated into cytosolic (left column) and
nuclear (right column) fractions. Expression levels of all five STAT proteins in either cytosolic or
nuclear extracts from EAE mice were equivalent to levels in control mice (P>0.05). Values are
presented as mean ± SEM.

Shindler et al.Immunology Innovation  2013 1:3DOI : 10.7243/2053-213X-1-3