Figure 8 : Protein was extracted from lymph nodes isolated from EAE mice 10 days post-immunization.

(A) Western blots of the nuclear fractions of protein extracts from lymph nodes of control (lanes 1–6, n=6 for STAT1, STAT3 and STAT4; lanes 1-5, n=5 for STAT5
and STAT6) and EAE (lanes 7–10, n=4 for STAT1, STAT3 and STAT4; lanes 6-10, n=5 for STAT5 and STAT6) mice. (B) There was a significant decrease (*P<0.01)
of expression of STAT3, STAT4 and STAT6 proteins in lymph nodes from EAE mice compared with lymph nodes from control mice. Differences in the level of
expression of STAT1 and STAT5 in lymph nodes from EAE mice as compared with levels in control mice were not significant (P>0.05). Values are presented as
mean ± SEM.

Shindler et al.Immunology Innovation  2013 1:3DOI : 10.7243/2053-213X-1-3