Figure 1 : Representative image of three independent western blot experiments for nuclear factor-kappa B p50
(NF-κB) and p38 mitogen-activated protein kinase (MAPK) evaluation.

Periodontal ligament cells (A) and osteoblastic rat cells (B) grown on six-well plates were deinduced for 12 h in culture medium
containing 0.3% fetal bovine serum and then stimulated with lipopolysaccharide from Escherichia coli or Porphyromonas gingivalis
(100 ng/mL and 1 μg/mL) or IL1β (10 ng/mL and 100 ng/mL) for 0 (control–C), 10, 30, 60 and 120 minutes. E. coli, Escherichia coli;
P. gingivalis, Porphyromonas gingivalis; IL-1b, interleukin-1 beta; GAPDH, glyceraldehyde-3-phosphate dehydrogenase;
LPS, lipopolysaccharide.

GuimarĂ£es et al.Immunology Innovation  2014 2:1DOI : 10.7243/2053-213X-2-1