Figure 3 : Time course of receptor activator of nuclear factor-kappa B ligand (RANKL) and osteoprotegerin (OPG)
mRNA expression induced by Escherichia coli (A), Porphyromonas gingivalis (B) and interleukin-1 beta (C) in osteoblastic
cells (Ros).

Bars indicate mean and standard deviation of densitometric analysis of three independent experiments. Data are expressed
as fold change in unstimulated control group (ctrl) after normalization to GAPDH. In all graphs asterisk (*) indicates
significant difference (p<0.05) in relation to ‘1’ attributed to unstimulated control (ctrl) by one-sample t test. Exclamation
mark (!) indicates significant difference (p<0.05) within the same time point with the other dose of the same agonist by
unpaired t test. E. coli, Escherichia coli ; P. gingivalis, Porphyromonas gingivalis ; IL-1b, interleukin-1 beta; LPS,
lipopolysaccharide; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.

GuimarĂ£es et al.Immunology Innovation  2014 2:1DOI : 10.7243/2053-213X-2-1