Figure 5 : Relevance of p38 MAPK and NF-kB for expression of RANKL and OPG mRNA upon activation of TLR2, TLR4
and IL1R in fibroblasts (A) and osteoblasts (B).

Bars indicate mean and standard deviation of densitometric analysis of three independent experiments. Data are
expressed as percent change (decrease) of normalized target gene expression when the stimulations were conducted
in the presence of pretreatment with specific inhibitors for p38 MAPK (SB203580) or NF-kB (Bay 11-0782). In all
graphs asterisk (*) indicates significant difference (p<0.05) in relation to ‘1’ attributed to unstimulated control by
one-sample t test. Exclamation mark (!) indicates significant difference (p<0.05) within the same dose comparing
24h and 48h stimulation with the same agonist by unpaired t test. E. coli, Escherichia coli ; P. gingivalis,
Porphyromonas gingivalis ; IL-1β, interleukin-1 beta; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.

GuimarĂ£es et al.Immunology Innovation  2014 2:1DOI : 10.7243/2053-213X-2-1