Figure 1 : CT26(hTERT) cell lines.

(a) Hygromycin B resistant hTERT-Flag2-IREShyg transfected CT26 (C) cell colonies were picked, and hTERT-Flag2 expression in cell lines #1, #2, #3, #4, #5, #6, #8, #10, and #12 was assessed using a monoclonal antibody M2 against the flag tag and a goat polyclonal anti-hTERT antibody (Santa Cruz,sc-7215). An anti-β-actin monoclonal antibody (abcam, ab8226) was used as a calibration control, and hTERT-Flag2, mTERT, and β-actin signals were indicated by red, black, and blue arrow heads, respectively.
(b) After removal of hygromycin B for 1 week, hTERT-Flag2 expression remained stable in CT26(hTERT #10) cells and was abolished in CT26(hTERT #4) cells.

Chuang et al.Journal of Immunotherapy Applications  2015 2:1DOI : 10.7243/2055-2394-2-1