Figure 4 : Effect of volasertib on the ATPase activity of (A) ABCB1 and (B) ABCG2.

The vanadate-sensitive ATPase activity of ABCB1 or ABCG2, in membrane protein obtained from the respective transporter-overexpressing High Five insect cells, was determined atdifferent concentrations of volasertib. ATP hydrolysis was monitored by measuring the amount of inorganic phosphate released using a colorimetric assay.

To et al.Journal of Cancer Therapeutics and Research  2013 2:13DOI : 10.7243/2049-7962-2-13