Journal of Cancer Therapeutics & Research

Journal of Cancer Therapeutics & Research

ISSN 2049-7962
Original Research

NH125, an eukaryotic elongation factor 2 kinase inhibitor, radiosensitizes breast cancer by the abrogation of G2/M checkpoint

Hong-Mei Song1†, Guang-Zong Chen3†, Hong-Cheng Zhu3†, Jian-Hua Ma2, Zhong-Ming Wang2, Tian-Li He2, Yuan-Dong Wang3, Xin-Chen Sun3 and Jian-Xin Ma4*

*Correspondence: Jian-Xin Ma

† These authors contributed equally to this work.

4. Department of Radiation Oncology, the Dongfang Hospital of Lianyungang, Lianyungang 222000, China.

Author Affiliations

1. Department of Radiation Oncology, the Central Hospital of Taian, Taian 271000, China.

2. Department of Radiation Oncology, Lianyungang NO.2 People’s Hospital Affiliated to Bengbu Medical College, Lianyungang 222000, China.

3. Department of Radiation Oncology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.


Radiotherapy remains an important treatment option for both surgical and non-surgical breast cancer patients; however resistance to radiation results in tumor recurrence and metastasis. eEF2K has been reported to play a critical role in cancer development and progression, but little is related to its radiosensitization effect. This study aimed to evaluate the radiosensitization effect of NH125, an eukaryotic elongation factor 2 kinase inhibitor, in breast cancer MDA-MB-231 and MCF-7. Cell proliferation rate was evaluated by Cell Counting Kit 8 assay. Cell cycle distribution and apoptosis rate were detected by flow cytometry. Radiosensitization ratio was evaluated by clonogenic survival assay. DNA damage repair was detected by immunofluorescent assay. Tumor-bearing xenografts confirmed the radiosensitization effect in vivo. NH125 potently sensitized MDA-MB-231 and MCF-7 to radiation with sensitization enhancement ratios 1.29 and 1.43 respectively. NH125 enhanced the radiosensitization through abrogation of G2 checkpoint and prolongation of DNA damage repair. Above all, these results demonstrated that NH125 may be a promising radiosensitizer in breast cancer radiotherapy.

Keywords: NH125, eEF2K, Eukaryotic elongation factor 2 kinase, Breast cancer, Radiosensitivity

ISSN 2049-7962
Volume 6
Abstract Download