2. Seventh People’s Hospital of Hebei Province, Urology Surgery, Jungong Road No.389, Baoding City, Hebei Province, China.
3. Cangzhou Central Hospital, Nephrology, Xinhua West Road No. 16, Cangzhou, Hebei Province, China.
Purpose: HOXB7 is a gene of HOX gene family, which has been found to play a role in cancer cell proliferation, angiogenesis, and invasion. Due to its potential responsibility in cancer aggression, the main objective of this work was to investigate the expression in Renal cell carcinoma and the biological functions in Renal cell carcinoma cells.
Methods: To investigate HOXB7 expression in Renal cell carcinoma tissues and Renal cell carcinoma cell lines by using immunohistochemistry, Western blot and RT-PCR. Then we analyzed the relationship between HOXB7 expression and Renal cell carcinoma clinical parameters. The biological function of HOXB7 on proliferation, migration and invasion were investigated by CCK8 assays, Transwell and Matrigel assays, after knocking down the HOXB7 expression in Renal cell carcinoma cells with siRNAs.
Results: The results showed that HOXB7 protein was highly expressed in the Renal cell carcinoma tissues and cell lines. Down-regulating HOXB7 expression by siRNAs could suppress the proliferation, migration and invasion in Renal cell carcinoma cells. The HOXB7 expression was related to the poor differentiation by statistical analysis. Besides, HOXB7 was related to the patient prognosis by the survival analysis.
Conclusions: HOXB7 protein was expressed in the Renal cell carcinoma, which was related to the poor prognosis in Renal cell carcinoma patients. HOXB7 promoted the proliferation, migration and invasion in Renal cell carcinoma cells. Therefore, it may be a new marker to predict the prognosis and target to cure Renal cell carcinoma.
Keywords: HOXB7, Renal cell carcinoma, prognosis, migration, invasion