Figure 1a-f: Transmission electron microscopy of 50μM ProIAPP1-48 in physiological medium at pH 7.4 following incubation for 24h at 37μC in the presence of various combinations of metals. a – ProIAPP1- 48 only; b – ProIAPP1-48 + 500 μM Al(III); c – ProIAPP1-48 + 500 μM Zn(II); d - ProIAPP1-48 + 50 μM Cu(II); e - ProIAPP1-48 + 500 μM Al(III) + 50 μM Cu(II); f - ProIAPP1-48 + 500 μM Zn(II) + 50 μM Cu(II). Asterisk identifies the position of the magnified insert. Each insert shows a fibril diameter in nm. Scale bars; a,b,e – 200 nm; c,d,f – 500 nm.

Exley et al.Journal of Diabetes Research and Clinical Metabolism  2012 1:3DOI : 10.7243/2050-0866-1-3