Cu(II)-ProIAPP1-48

0

0

1:1

1:1

5:1

5:1

 

 

+Cu(II)

 

+EDTA

 

+EDTA

ProIAPP1-48        0h

                 24h

                 72h

  29(10.9)

  81(19.6)

216(21.9)

 

  40(  2.5)

  41(  8.8)

 

  5(  1.6)

  6(  4.2)

  8(  3.8)

 

60(17.7)

72(24.4)

0

0

0

 

30(  7.7)

26(  5.9)

ProIAPP1-48        0h

+Al(III)             24h 

                 72h

  29(16.8)

177(85.5)

230(71.9)

 

138(92.1)

  91(50.7)

13(  5.0)

  3(  1.4)

11(16.0)

 

65(31.2)

76(24.8)

3(4.6)

0

0

 

36(13.1)

25(  3.5)

 

ProIAPP1-48    0h

+ Zn(II)             24h

               72h

  11(  5.5)

  30(  6.0)

  83(17.4)

 

  25(  9.7)

  31(  9.4)

  0

  1(  0.9)

  1(  2.0)

 

52(14.3)

78(33.0)

0

0

0

 

21(  6.1)

21(  4.6)

 

 Table 3. The influence of equimolar and 5-fold excess Cu(II) on ThT fluorescence of 50 mM ProIAPP1-48 in the absence and presence of a 10-fold excess of Al(III) or Zn(II) after 0, 24 and 72h incubation at 37°C.  After 24 and 72h either Cu(II) (to give 200 mM) or EDTA (to give 1 mM) was added to each preparation and ThT fluorescence measured after 150s. Mean and SD are given, n=3.

Exley et al.Journal of Diabetes Research and Clinical Metabolism  2012 1:3DOI : 10.7243/2050-0866-1-3