|
Cu(II)-ProIAPP1-48 |
0 |
0 |
1:1 |
1:1 |
5:1 |
5:1 |
|
|
|
+Cu(II) |
|
+EDTA |
|
+EDTA |
|
ProIAPP1-48 0h 24h 72h |
29(10.9) 81(19.6) 216(21.9) |
40( 2.5) 41( 8.8)
|
5( 1.6) 6( 4.2) 8( 3.8) |
60(17.7) 72(24.4) |
0 0 0 |
30( 7.7) 26( 5.9) |
|
ProIAPP1-48 0h +Al(III) 24h 72h |
29(16.8) 177(85.5) 230(71.9) |
138(92.1) 91(50.7) |
13( 5.0) 3( 1.4) 11(16.0) |
65(31.2) 76(24.8) |
3(4.6) 0 0 |
36(13.1) 25( 3.5)
|
|
ProIAPP1-48 0h + Zn(II) 24h 72h |
11( 5.5) 30( 6.0) 83(17.4) |
25( 9.7) 31( 9.4) |
0 1( 0.9) 1( 2.0) |
52(14.3) 78(33.0) |
0 0 0 |
21( 6.1) 21( 4.6)
|
Table 3. The influence of equimolar and 5-fold excess Cu(II) on ThT fluorescence of 50 mM ProIAPP1-48 in the absence and presence of a 10-fold excess of Al(III) or Zn(II) after 0, 24 and 72h incubation at 37°C. After 24 and 72h either Cu(II) (to give 200 mM) or EDTA (to give 1 mM) was added to each preparation and ThT fluorescence measured after 150s. Mean and SD are given, n=3.