Table 5 : Retention times of each individual amino
acid in GC/FID analysis*.


Amino acid Retention time (min)

Alanine 13.291
Glycine 13.496
Valine 14.629
Leucine 15.340
Isoleucine 15.554
Norleucine (the internal standard) 15.730
Proline 15.934
Methionine 17.835
Serine 18.123
Threonine 18.381-18.477
Phenylalanine 19.007
Aspartic acid 19.627
Hydroxy Proline 19.816
Glutamic acid 20.566
Asparagine 21.042
Lysine 21.450
Glutamine 21.926
Tryptophan 21.983-22.113
Arginine 22.255
Histidine 23.132
Tyrosine 23.535
Cysteine 27.132

*Derivation of amino acids were conducted derived with
the derivatization reagent MTBSTFA (N-Methyl-N-
(Tert-Butyldimethylsilyl)trifluoroacetamide in pyridine.

Chen et al.Journal of Diabetes Research and Clinical Metabolism  2014 3:5DOI : 10.7243/2050-0866-3-5