Figure 2 : Expression analysis by RT-PCR of ggpPS in transgenic plants. RT-PCR of ggpPS fragments obtained with total leaf RNA from wild type plants and different transgenic plants. Specific primer combinations for amplification of ggpPS and 60S RNA (loading control) were used. 1 – RNA from young leaf of Alba-AF1, 2 –RNA from old leaf of Alba-AF1, 3 – RNA from stolon of Alba- AF1, 4 - RNA from tuber of Alba-AF1, 5 - RNA from young leaf of wild type, 6 – RNA of young leaf of Alba- AF1 with heat-inactivated reverse transcriptase, 7 – DNA
of transformation vector pLH-AF.

Hagemann et al.Journal of Plant Science and Molecular Breeding  2013 2:1DOI : 10.7243/2050-2389-2-1