Table 1 : Genetic, marker and phenotypic assessment of mutations compromised for Yr5-mediated yellow rust resistance.


Varieties, Yr5del &
Yr5EMS mutants
Yr5EMS
IT to WYR
81/20
Yr5EMS mutants x AvocetS:1
Yr5 infection phenotypes
DNA marker analysis: Alleles present2
    F1 IT F2 segregating ITs STS: S19M93
-100
STS: S23M41
-275
GWM
120
WMC
175
GWM
526
TaAffx.
65234.1
S1_at
Ta28038
n; 0n/nn 1/2n 3/4
Yr5EMS 18 1n 4 0 1 19 214 T.sp T.sp T.sp T.sp T.sp T.sp T.sp
Yr5EMS 90 4 4 0 6 15 189 T.sp T.sp T.sp T.sp T.sp T.sp T.sp
Yr5EMS 94 0nn 0nn 27 66 39 84 T.sp T.sp T.sp T.sp T.sp T.sp T.sp
Yr5EMS 95 4 4 0 0 0 234 T.sp T.sp T.sp T.sp T.sp T.sp T.sp
Yr5EMS 98 1/2n 4 0 34 66 122 T.sp T.sp T.sp T.sp T.sp T.sp T.sp
Yr5EMS 99 4 4 0 0 12 218 T.sp T.sp T.sp T.sp T.sp T.sp T.sp
Yr5EMS 115 4 4 0 0 0 228 T.sp T.sp T.sp T.sp T.sp T.sp T.sp
Yr5EMS 241 4 4 0 0 0 212 T.sp T.sp T.sp T.sp T.sp T.sp T.sp
Yr5EMS 287 4 4 0 0 0 216 T.sp T.sp T.sp T.sp T.sp T.sp T.sp
Yr5EMS 387 4 4 0 0 0 220 T.sp T.sp T.sp T.sp T.sp T.sp T.sp
Yr5EMS 474 4 4 0 0 0 218 T.sp T.sp T.sp T.sp T.sp T.sp T.sp
Yr5EMS 500 4 4 0 0 0 228 T.sp T.sp T.sp T.sp T.sp T.sp T.sp
Yr5del55 4 - - - - - DEL DEL T.sp T.sp T.sp DEL DEL
Yr5del67 4 - - - - - DEL DEL T.sp T.sp T.sp DEL DEL

Controls:                          

Lemhi*8/Yr5 n; 0n/1n 52 116 2 64 T.sp T.sp T.sp T.sp T.sp T.sp T.sp
Triticum spelta ; - - - - - T.sp T.sp T.sp T.sp T.sp T.sp T.sp
Lemhi 4 - - - - - null L L L L L L
Avocet S 4 - - - - - n/t n/t n/t n/t n/t n/t n/t
Chinese Spring 3/4 - - - - - null L CS CS CS n/t n/t

1Lemhi*8/Yr5 and the Yr5EMS mutants were crossed to the yellow rust susceptible variety AvocetS. The infection type (IT) scores given to the F1 and F2 progeny
follow the scheme set out in Materials and Methods.
2Marker alleles are indicated as T.sp. (as present in Triticum aestivum ssp. spelta var. album), CS (as present in cultivar Chinese Spring), L (as present in cultivar Lemhi),
null (no band detected), or DEL (marker absent), n/t (not tested).

Boyd et al.Journal of Plant Science and Molecular Breeding  2014 3:2DOI : 10.7243/2050-2389-3-2