Table 3 : PCR analysis of Affymetrix Wheat1 GeneChip probe sets down-regulated in Yr5del55 and
Yr5del67 compared to Lemhi*8/Yr5.


  qRT-PCR a End point RT-PCR PCR from genomic DNA

Probe Set ID Yr5del55 Yr5del67 Yr5del55 Yr5del67 Yr5del55 Yr5del67

Ta.1944.1.S1_at 33.5 -23.7 + b + + +
Ta.27140.1.S1_at nt d nt - - + +
Ta.178.2.S1_at/ Ta.178.2.S1_x_at -2194.5 -8364.0 - - - -
Ta.1688.1.S1_at -106.7 -56.4 + + + +
Ta.14545.1.S1_at -1294.9 -1956.8 - - + +
Ta.1112.1.S1_at -3.1 -0.9 + + + +
TaAffx.14477.1.S1_at nt nt + + + +
Ta.611.1.A1_at -42912.3 -21240 - - + +
Ta.21290.1.A1_s_at -4.3 -2.7 + + + +
Ta.8757.2.S1_at -4.4 -4.6 + + + +
Ta.21005.1.S1_at -83.8 -271.3 - - + +
Ta.14729.1.S1_at 2.8 -1.0 + + + +
Ta.30504.1.A1_at -584.8 -253.0 - - + -
TaAffx.73741.1.S1_at nt nt - - + +
Ta.17028.1.A1_s_at nt nt + + + +
Ta.7149.1.A1_s_at nt nt + + + +
TaAffx.616.2.S1_s_at 0.0 -2.2 + + + +
TaAffx.66205.2.S1_s_at nt nt + + + +
TaAffx.100436.1.S1_at nt nt - - - -
Ta.16298.1.S1_at nt nt + + + +
Ta.16582.1.S1_at nt nt + - + +
Ta.8552.1.A1_at -231.5 -154.1 - - + +
Ta.16476.1.S1_at -2.3 -0.7 + + + +
TaAffx.43914.1.S1_s_at nt nt - - + +
Ta.17295.1.S1_at nt nt - - - -
Ta.9039.2.S1_x_at -13.0 -11.9 + + + +
TaAffx.53602.1.S1_at nt nt nt nt nt nt
TaAffx.57738.1.S1_at nt nt + + + +
TaAffx.51578.1.S1_at nt nt nt nt nt nt
TaAffx.7104.1.S1_at/
TaAffx.7104.1.S1_x_at
nt nt nt nt nt nt
TaAffx.74512.2.S1_s_at -530.9 -300.0 - - - -
Ta.611.2.S1_at -28521.9 -10866.1 - - + +
TaAffx.21788.1.S1_at nt nt - - + +
Ta.19222.1.S1_at/
Ta.19222.1.S1_x_at
-1.1 -10.8 + + + +
Ta.20262.2.S1_at nt nt + + + +
Ta.23203.3.S1_at nt nt nt nt nt nt
TaAffx.22603.1.S1_at 0.0 -1.3 + + + +

aTranscript levels expressed as fold change differences in deletion mutant relative to Lemhi*8/ Yr5. Underlined
expression values highlight those probe sets where qRT-PCR analysis was used to validated the microarray data.
b+ = PCR amplicon detected in mutant
c- = PCR amplicon absent from mutant
dnt = not tested

Boyd et al.Journal of Plant Science and Molecular Breeding  2014 3:2DOI : 10.7243/2050-2389-3-2