Figure 9 : PCR using the purified PCR product ppk PCR product as template.

L was the DNA ladder(DM10000, Beijing ComWin Biotech Co., Ltd, Cat: CW0634A). The molecular weight of the DNA band in DNA ladder was 10 kb, 7 kb, 4 kb, 2 kb, 1 kb, 500 bp, and 250 bp respectively. 1 referred to the ppk PCR product using common PCR procedure. 2 was the ppk PCR product using gradient PCR procedure.

Haiyan et al.Journal of Plant Science and Molecular Breeding  2016 5:2DOI : 10.7243/2050-2389-5-2