Journal of Plant Science & Molecular Breeding

Journal of Plant Science & Molecular Breeding

ISSN 2050-2389
Original Research

The First Genetic Map and Positions of Major Fruit Trait Loci of Bitter Melon (Momordica charantia)

Chittaranjan Kole1*, Bode A. Olukolu2, Phullara Kole1, Virendra K. Rao3, Anju Bajpai4, S. Backiyarani5, Jogendra Singh6, R. Elanchezhian7 and Albert G. Abbott1

*Corresponding author: Chittaranjan Kole ckole@clemson.edu

1. Department of Genetics & Biochemistry, 109 Jordan Hall, Clemson University, Clemson, USA.


Author Affiliations

2. Department of Plant Pathology, North Carolina State University, Raleigh NC 27695, USA.

3. G.B. Pant University of Agriculture & Technology, Ranichauri, Uttarakhand 249199, India.

4. Central Institute for Subtropical Horticulture, Lucknow, Uttar Pradesh 227107, India.

5. National Research Center for Banana, Trichy, Tamil Nadu 620102, India.

6. ICAR Research Complex for NEH, Imphal, Manipur 795004, India.

7. ICAR Research Complex for Eastern Region, Patna, Bihar 800014, India.

Abstract

Albeit extensive cultivation of bitter melon both as vegetable and medicine in many countries of Asia, Africa, and South America, no serious efforts have been made for genetic and breeding studies on this 'orphan' crop. In contrast to popular cucurbits, it lacks a genetic linkage map as required for genomic depiction and precise breeding. We report here on the construction of the first genetic linkage map of bitter melon using a set of 146 F2 progenies derived from an inter-botanical variety cross between Taiwan White, Momordica charantia var. charantia, and CBM12, M. charantia var. muricata. This map consists of 108 AFLP markers and five qualitative trait loci dispersed over 11 linkage groups spanning a total distance of 3060.7 cM. The five qualitative traits mapped include fruit color, fruit luster, fruit surface structure, stigma color, and seed color; all of which exhibited monogenic segregation except seed color which showed digenic (9:7) mode of inheritance. Besides, twelve quantitative trait loci (QTL) controlling five polygenic fruit traits including length, diameter, weight, number, and yield were detected on five linkage groups that individually explained 11.1 to 39.7% of the corresponding total phenotypic variance. This map will be useful in marker-assisted breeding of these fruit traits and future mapping of genes/QTLs controlling phytomedicines content exhibiting contrasting variation between the parents.

ISSN 2050-2389
Volume 1
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