Journal of Plant Science & Molecular Breeding

Journal of Plant Science & Molecular Breeding

ISSN 2050-2389

Behind the scenes of microspore-based double haploid development in Brassica napus: A review

Mukhlesur Rahman* and Monika Michalak de Jiménez

*Correspondence: Mukhlesur Rahman

Author Affiliations

Department of Plant Sciences, North Dakota State University, Fargo, ND 58108, USA.


Double haploids are extremely valuable for generating completely homozygous genotypes and have been used in plant breeding program of a number of crop species. This method is a much faster way of developing genetically pure breeding lines in one single generation. The main objective of this review is to describe in a clear and simple manner how microspore-based double haploids of rapeseed are produced and helps a reader to understand the amazing process of microspore embryogenesis, also referred to as androgenesis or pollen embryogenesis. This review will explain what double haploids are as well as their importance in both Brassica breeding and molecular studies. Additionally, a brief discussion of different factors affecting the double haploid production and a comprehensive explanation of the steps involved in the development of the double haploids will be covered.

Keywords: Double haploid, protocol, Brassica, plant breeding

ISSN 2050-2389
Volume 5
Abstract Download