Table 1 : Preparation of spiked plasma calibration standards.


Final Concentration,
ng/ml
Volume of Spiked Plasma,
µl (Stock Vial Used)
Volume of Blank Plasma, µl Stock Vial

5 200 (I) 200 J
10 50 (F) 450 I
25 200 (G) 200 H
50 200 (F) 200 G
100 50 (C) 450 F
250 200 (D) 200 E
500 200 (C) 200 D
1000 50 (B) 450 C

Woo et al.Journal of Pharmaceutical Technology and Drug Research  2017 6:2DOI : 10.7243/2050-120X-6-2