Figure 2 : The in vitro neuroectoderm-derived hESC-I hNuPs retain an embryonic acetylated globally active chromatin.


The strong expression and nuclear localization of a set of active chromatin modifiers in hESC-I hNuPs, compared to hESC-D and CNS-D hNSCs, including acetylated histones H3 and H4 (acH3 and acH4), HDAC1,chromatin-remodeling factors Brg-1 and hSNF2H. DAPI stains nuclei. Quantitative intracellular imaging analysis shows that the prototypical neuroepithelial-like hNSCs either derived from hESCs or the fetal CNS tissue display decreasing histone H3 acetylation (~ 2 fold) and decreasing histone H4 acetylation (~ 3-6 fold), despite strong expression of active chromatin-remodeling factors HDAC1, Brg-1, and hSNF2H. The expression levels of these active chromatin-modifying molecules in these human stem cell derivatives were quantified and plotted as relative levels in comparison to their expression levels in pluripotent hESCs. The expression levels of these active chromosomal proteins in pluripotent hESCs are set at 100%. Scale bars: 5 μm. Quantification data are presented as Mean ± SD; Statistical significance was defined as *P < 0.05, **P < 0.01.

Xuejun Parsons Journal of Regenerative Medicine and Tissue Engineering  2012 1:3DOI : 10.7243/2050-1218-1-3