Figure 6 : The neuroectodermderived hESC neuronal lineagespecific derivative is highly neurogenic in vitro and in vivo.

(A). Under neuronal differentiation conditions, hESC-I hNuPs yielded exclusively neurons that expressed neuronal marker β-III-tubulin and co-expressed Map-2 with a drastic increase in efficiency when compared to similarly cultured cells derived from un treated embryoid bodies (EBs) as the control. No cell expressed glial lineage marker GFAP and MBP. Accordingly, a large proportion of these hESC-derived neuronal cells began to express tyrosine hydroxylase (TH).

(B). hESC-I hNuPs were injected into the cerebral ventricles of newborn mice. Histological analysis of transplanted mice showed welldispersed and well-integrated human neurons at a high prevalence, indicated by anti-human mitochondrial antibody (hMit) and their immunoreactivity to Map-2, including Nurr1-positve and TH-positive DA neurons, within neurogenic regions of the brain. DAPI nuclear marker stains all cells in the field.

Xuejun Parsons Journal of Regenerative Medicine and Tissue Engineering  2012 1:3DOI : 10.7243/2050-1218-1-3