Journal of Regenerative Medicine & Tissue Engineering

Journal of Regenerative Medicine & Tissue Engineering

ISSN 2050-1218
Original Research

Chitosan-collagen scaffolds can regulate the biological activities of adipose mesenchymal stem cells for tissue engineering

Idiberto José Zotarelli Filho1,2*, Luiz Fernando Frascino2, Oswaldo Tadeu Greco2, José Dalmo de Araújo2, Aldemir Bilaqui2, Elias Naim Kassis3, Roberto Vito Ardito2 and Gustavo O. Bonilla-Rodriguez1

*Correspondence: Idiberto José Zotarelli Filho

1. State University of São Paulo-IBILCE-UNESP, Rua Cristovão Colombo 2265, São José do Rio Preto SP Brazil.

Author Affiliations

2. Institute of Cardiovascular Diseases-IMC-Rua Castelo D´Agua 3030-São José do Rio Preto SP Brazil.

3. Institute of Mouth-São José do Rio Preto/SP, Brazil.


Scaffolds of chitosan and collagen can offer a biological niche for the growth of adipose derived stem cells (ADSC). The objective of this work was to characterize the physico-chemical properties of the scaffolds and the ADSC, as well as their interactions to direct influences of the scaffolds on the behavior of ADSC. The methodology included an enzymatic treatment of fat obtained by liposuction by collagenase, ASDC immunophenotyping, cell growth kinetics, biocompatibility studies of the scaffolds analyzed by the activity of alkaline phosphatase (AP), nitric oxide (NO) determination by the Griess-Saltzman reaction, and images of both optical and scanning electron microscopy of the matrices. The extent of the crosslinking of genipin and glutaraldehyde was evaluated by ninhydrin assays, solubility tests and degradation of the matrices. The results showed that the matrices are biocompatible, exhibit physical and chemical properties needed to house cells in vivo and are strong stimulators of signaling proteins (AP) and other molecules (NO) which are important in tissue healing. Therefore, the matrices provide a biological niche for ADSC adhesion, proliferation and cells activities.

Keywords: Chitosan-collagen-genipin scaffolds, ADSC, macrophages, biological niche

ISSN 2050-1218
Volume 2
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