Microbiology Discovery

Microbiology Discovery

ISSN 2052-6180
Original Research

Growth of the Desulfomicrobium sp. strains, their sulfate- and lactate usage, production of sulfide and acetate by the strains isolated from the human large intestine

Ivan V. Kushkevych

Correspondence: Ivan V. Kushkevych ivan.kushkevych@gmail.com

Author Affiliations

Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho 1/3, CZ-61242 Brno, Czech Republic.


The aim and background of the study were to investigate bacterial growth of the Desulfomicrobium sp. strains from human large intestine, and to study the processes usage of sulfate- and lactate, production of sulfide and acetate by the obtained bacteria as well as to carry out cluster and correlation analysis of these processes. Microbiology methods of the study for bacterial strains cultivation and photometric methods for determination of bacterial biomass and hydrogen sulfide concentration were used, sulfate ions concentration was determined by turbidymetric method, lactate concentration was carried out by lactate dehydrogenase. Acetate ions accumulation by the strains was determined by titration. Using the experimental data, the methods of statistical analysis have been also used. The highest biomass (3.34 g/l) has been accumulated by the Desulfomicrobium sp. strain Rod-9 on the sixth day of cultivation, in comparison to the other strains. Studied strains of the Desulfomicrobium sp. actively reduced the sulfate ions and produced hydrogen sulfide. The highest concentration of hydrogen sulfide (3.14 mM) was produced by the SRB Rod-9 strain on the eighth day of cultivation; while, the strain used about 98% of the sulfate compared with its initial concentration. The strains used lactate fully on the sixth day of cultivation and produced acetate. Clustering of the parameters of the bacterial growth, the sulfate and lactate usage as well as hydrogen sulfide and acetate production by the various Desulfomicrobium sp. strains has been carried out. The correlation coefficients between the parameters have been determined.

Keywords: Sulfate-reducing bacteria, Desulfomicrobium , intestinal microbiocenosis, sulfates, sulfide, inflammatory bowel diseases

ISSN 2052-6180
Volume 2
Abstract Download