2. Biotechnology Department, Faculty of Science, Taif University, Kingdom of Saudi Arabia.
Aim: The study is aimed to detect the presence of cagA, iceA1, and iceA2 virulence genes in H. pylori from gastric biopsies, and to deduce the correlation between these genotypes and the two clinical outcomes peptic ulcer disease (PUD), and gastritis.
Materials and methods: Thirty three Saudi patients 15 males and 18 females, 20 to 90 years were assigned into two groups PUD and gastritis. Genomic DNAs were extracted from biopsy specimens and used to detect the presence of cagA, and iceA genes by PCR typing system. Fisher's and Phi coefficient association tests were used for statistical analysis.
Results: Genotyping show that both cagA and iceA genes were amplified from 27 specimens (81.7%). The prevalence of cagA+ and cagA- genotypes or iceA+ and iceA- genotypes did not differ significantly between males and females (p=0.070). Within the PUD and gastritis groups, the percentages of specimens positive for cagA gene were 76.9 % and 85 %, while those positive for iceA+ were 92.3% and 75% respectively. All cagA+/iceA+ combined genotypes was statically correlated with peptic ulcer (77%). This correlation was not observed within H. pylori specimens typed from gastritis. Patients with either PUD or gastritis were most likely infected by several strains of H. pylori.
Conclusion: Different strains of H. pylori have virulent genotypes evidenced by PCR-based genotyping from biopsy specimens at a reasonable cost and time. These virulence strains spread at Taif province, may result in sever clinical outcomes such as ulcers which may be developed to cancer, the situation which necessitates further studies.
Keywords: H. pylori, cagA genotype, iceA+ genotype, peptic ulcer, gastritis, PCR-based genotyping, gastric biopsies