Figure 4 : Domain Specific Recruitment of PPP2R3C complexes and De-phosphorylation of CREB-P: Preimmune IgG served as control to define the specificity of CREB-P IgGs and protein complexes analyzed by SDSPAGE. Immunoprecipitated actin in extracts was used as control in standardizing. Lane 1 contains control extract with pre-immune IgGs. Lanes 2-9 contains extracts from pCMV PPP2R3C co-transfected cells. Lanes 3, 5, 7 & 9 contain extracts treated with 100nM DEX. Lanes 2, 4, 6 & 8 contain extracts from non-ligand treated transfectants. Lanes 2 & 3 contain extracts from control pCMV vector transfected cells. Lanes 4 & 5 contain hGRwt, lanes 6 & 7 contain GR S113A and lanes 8 & 9 contain GR C638S cotransfected cells respectively.





Govindan et al.Molecular Biology and Genetic Engineering  2013 1:2DOI : 10.7243/2053-5767-1-2