Figure 7 : A. Knockdown and Recue of PPP2R3C: For knockdown and rescue of PPP2R3C in HeLa cells, whole cell extracts were prepared from control HeLa and G418 selected PPP2R3C knockdown HeLa cells from two different colonies, A and B. Control vector selected HeLa cells served as negative control. Actin was used to monitor level of identical amounts of protein. Cell extracts were prepared as described used in Western blotting with antibodies against GR, PPP2R3C and actin. B. Immunoblotting of GR and PPP2R3c in Knockdown cells: 35S Methionine labeled cell extracts from control, PPP2R3C knockdown A and B, and PPP2R3C rescued knockdown cells were analyzed by immunoprecipitation using GR IgGs or control anti-actin IgGs. Rescue of PPP2R3C knockdown A and PPP2R3C knockdown B cells was by introduction of pCMV Sport6 PPP2R3C (lanes 5 & 6).



Govindan et al.Molecular Biology and Genetic Engineering  2013 1:2DOI : 10.7243/2053-5767-1-2