Figure 8 : PPP2R3C Knockdown and Analysis of GR Feedback regulation of GR gene using FBRE-tk Luciferase reporter: Analysis of feedback regulation of GR gene in PPP2R3C Knockdown a HeLa cells and rescue by introduction of pcDNA 1 PPP2R3C with control vector pCMV β-galactosidase.The transfectants were treated with vehicle or ligands as indicated. The whole cell extracts were prepared; β-galactosidase activities were determined in 10μl of the total extracts. Luciferase activities were determined in extracts containing 10U of β-galactosidase. The results are the mean of three independent experiments.



Govindan et al.Molecular Biology and Genetic Engineering  2013 1:2DOI : 10.7243/2053-5767-1-2