Figure 2 : Transformation and regeneration of transformed sorghum plants and GUS Histochemical assay.

(A). Shoot apex explants of sorghum cultivar APK 1 (Sorghum bicolor L. cv. APK1) after infection with Agrobacterium tumefaciens LBA4404 (pCAMBIA1305.1/ Cry1C).
(B). Hygromycin resistant calli of the sorghum cultivar (Sorghum bicolor L. cv. APK1) after 42 days of cocultivation in selection medium.
(C). The transient expression of GUS gene in the calli of sorghum cultivar APK 1 (Sorghum bicolor L. cv. APK1) after cocultivation with Agrobacterium tumefaciens
LBA4404 (pCAMBIA1305.1) for ten days.
(D). Hygromycin resistant embryogenic calli of sorghum (Sorghum bicolor L. cv. APK1) on regeneration medium.
(E). The proliferation of hygromycin resistant shoots of the sorghum (Sorghum bicolor L. cv. APK1) after further subculture to selection medium.
(F). Proliferation of hygromycin resistant plants of the sorghum cultivar (Sorghum bicolor L. cv. APK1) after further subculture on regeneration medium.
(G). Rooting of hygromycin resistant plants of the sorghum cultivar (Sorghum bicolor L. cv. APK1) on rooting medium.
(H). Transformed sorghum (Sorghum bicolor L. cv. APK1) plants at in vivo conditions.
(I). Panicle of transformed sorghum (Sorghum bicolor L. cv. APK1) plants.

Savarimuthu et al.Molecular Biology and Genetic Engineering  2014 2:1DOI : 10.7243/2053-5767-2-1