Figure 1 : In silico experimental strategy to construct and analyze DNA pools using prGWAS on the
WTCCC data.

In the Collection stage, the 58C and NBS control cohorts are combined into a single control cohort comprising n~3000
individuals. Further, in the Master plates stage, the DNA master plates for the control and case cohorts are constructed
by randomly sampling without replacement ni individuals from each cohort (i=1,2). Likewise, up to k random samples
ni'=[ni/2] are subsequently draw from each master plates in the Subsampling stage (here, [x] the integer part of x). Finally,
in the Testing stage, the allele frequencies of m SNPs in cases and controls are compared for each pair of DNA pools, and
the P-values combined across all pools using meta-analytical methods.

Arcos-Burgos et al.Molecular Biology and Genetic Engineering  2015 3:1DOI : 10.7243/2053-5767-3-1