2. Department of Plant Molecular Biology, Delhi University South Campus, New Delhi, India.
3. Department of Biochemistry and Molecular Biology, Federal University of Ceara, Fortaleza, Brazil.
Background: Proteins isolated from the latex (LP) of Calotropis procera (family: Apocynaceae) have been shown to ameliorate inflammatory hyperalgesia in the rat model of Freund's Complete Adjuvant (FCA) induced monoarthritis. The objective of the present study was to evaluate the effect of LP on inflammation index and time of functional recovery in arthritic rats.
Methods: Monoarthritis was induced by intra-articular injection of FCA and joint diameter, motility, stair climbing ability and dorsal flexion pain scores were recorded before inducing arthritis and thereafter at different time intervals till joint functions recovered (day 16). The area under curve (AUC) for increase in joint diameter was calculated. The rats were sacrificed and the tissue levels of reduced glutathione and thiobarbituric acid reactive substances were measured. Histological analysis of the joint was also carried out. The effect of LP (5 and 25 mg/kg) and diclofenac (5 mg/kg) given on alternate days was evaluated on the parameters mentioned.
Results: LP produced a dose-dependent reduction in AUC for increase in joint diameter and the time required to attain the normal joint functions as compared to the arthritic controls. Treatment with LP normalized the levels of oxidative stress markers and preserved tissue architecture. The protection afforded by LP was comparable to that with diclofenac.
Conclusion: The present study shows that by inhibiting inflammation, LP fraction of latex of C. procera ameliorates functional limitations in arthritic rats.
Keywords: Arthritis, C. procera, functional limitations, inflammation, oxidative stress