Figure 4 : (A) Effect of concentration of beads upon maximum decrease in monomer signal using 3 μm avidin modified beads, binding capacity of 14 μg/mg, 25 min assay time and 5 x 108 beads /ml = red, 2.5 x 108 beads /ml = purple, 1 x 108 beads /ml = blue an 1 x 107 beads /ml = orange. Highlighted on the graph is the minimum in each curve for the dilutions. (B) Effect of concentration of beads upon maximum decrease in monomer signal, 25 min assay: 3 μm avidin modified beads 5 x 108 beads /ml, binding capacity 14 μg/mg = blue, 1 μm avidin modified beads, 6 x 109 beads /ml, binding capacity 20 μg/mg = red, and 1 μm avidin modified beads, 5 x 108 beads /ml binding capacity 20 μg/mg = green.

Platt et al.Nanomaterials and Nanosciences  2013 1:1DOI : 10.7243/2053-0927-1-1