Oral Biology and Dentistry

Oral Biology and Dentistry

ISSN 2053-5775
Original Research

Toxicity of root canal sealers in vitro

Anil Chandra1†, Hena Rahman2*, Amita Agarwal3† and Arun Verma4†

*Correspondence: Hena Rahman henavns@gmail.com

These authors contributed equally to this work.

1. Department of Conservative Dentistry and Endodontics, Faculty of Dental Sciences, King George's Medical University, Lucknow, India.

Author Affiliations

2. Department of Conservative Dentistry and Endodontics, Career Post Graduate Institute of Dental Sciences and Hospital, Lucknow, India.

3. Department of Pedodontics and Preventive Dentistry, Saraswati Dental College, Lucknow, India.

4. Department of Conservative Dentistry and Endodontics, Sardar Patel Post Graduate Institute of Dental and Medical Sciences, Lucknow, India.

Abstract

Objective: To comparatively evaluate the cytotoxicity of EndoREZ (urethane dimethacrylate based), RoekoSEAL (silicone based) and Kalsogen Plus (ZOE based) sealers on HEK-293 (human embryonic kidney epithelial cells) and Vero (African green monkey kidney epithelial cells) cell lines in vitro.

Materials and methods: Sealers EndoREZ, RoekoSEAL and Kalsogen Plus were mixed according to manufacturer's instructions. Extract of these sealers were then treated with cultured cell (HEK-293 and Vero), cytotoxicity evaluated using MTT assay. Cytotoxicity between fresh and set specimens at 0h, 24h, 48h, 72h and 1wk time-interval were calculated. Statistical analysis was done using SPSS Version 15.0 (SPSS, Inc., Chicago, IL, USA) statistical software package (p<0.05).

Results: Extracts of Kalsogen Plus were more toxic, as compared to all other groups (p<0.05) on both cell lines. The mean value of EndoREZ was significantly lower as compared to all other groups on both cell lines. The mean value of RoekoSEAL was significantly higher as compared to EndoREZ but significantly lower as compared to Kalsogen Plus.

Conclusion: Kalsogen Plus and RoekoSEAL were slightly cytotoxic while EndoREZ was non-cytotoxic on both cell lines. The cytotoxic response decreased in the order of Kalsogen Plus>RoekoSEAL>EndoREZ overtime.

Keywords: Toxicity, root canal sealers, MTT assay, inhibition

ISSN 2053-5775
Volume 2
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