2. Department of Restorative Dentistry, King's College London Dental Institute at Guy's, UK.
3. St Thomas' Hospital, Floor 25, Tower Wing, Guy's Hospital, St Thomas' St, London SE1 9RT, UK.
4. Institute for Genetic Engineering and Biotechnology, University of Sarajevo, Zmaja od Bosne 8, Kampus, 71000 Sarajevo, Bosnia and Herzegovina.
5. The Forsyth Institute, 245 First Street, Cambridge MA 02142, USA.
6. Harvard School of Dental Medicine, 188 Longwood Avenue, Boston MA, 02115, USA.
Objectives: This study investigated the correlation between Tannerella forsythia, Porphyromonas gingivalis, Fusobacterium nucleatum and Aggregatibacter actinomycetemcomitans at dual sites in concurrent endodontic-periodontal diseases.
Material and methods: Samples were collected from endodontium and periodontium in cases of concurrent endodontic-periodontal diseases from thirty participants. The sensitivity and specificity of SYBR Green real-time PCR was used to identify the targeted species. Absolute number of targeted genome copies in tested samples were extrapolated from respective calibration curve.
Results: No statistical difference was found in the number of detected endodonticperiodontal pathogens between the endodontium and periodontium. The Pearson test detected significant correlation (P<0.001) between targeted bacteria; T. forsythia, F. nucleatum, and P. gingivalis from endodontic-periodontal lesions. Synergistic component observed separately in endodontic biofilm was found only between T. forsythia and F. nucleatum (r=0.380, P=0.03) while in periodontal biofilm T. forsythia, F. nucleatum and P. gingivalis gave high synergism result (P<0.0001). Correlation analysis showed that T. forsythia in primary endodontic infection and in periodontal lesion was significantly decreased with the increase of patients age (r=-0.308, P=0.017).
Conclusions: Correlation between targeted bacterial species levels from concurrent endodontic-periodontal diseases confirmed that coronal and cervical dentinal tubules may represent a viable pathway that allows spreading and maintaining of dual sites infection. Periodontal bacteria detected in root canal of concurrent endodonticperiodontal infections may originate from the local periodontal lesions.
Keywords: Microbiome correlation, dentinal tubules, endo-perio infection, pearson test, periodontal lesion