Table 2 : Effect of Butea monosperma L. leaves extracts/Ciprofloxacin against adult filarial parasite Setaria cervi for 24 hrs
incubation in terms of MTT reduction assay. Results shown are mean±SEM of percent reduction.


Sample Treatment Test concentration
(mgmL-1)
Incubation
time (In hrs.)
Absorbance at 492
nm (mean ± s.e.m.)
% reduction relative to solvent controlC, heat killedH & treated wormsT IC50 (mgmL-1)

Methanol extract CControl
HHeat killed
TPlant extract
--
--
0.25
0.50
1.00
2.00
5.00
24
0.5
24.0
20.0
15.0
10.0
5.0
1.006 ± 0.003
0.319 ± 0.004
0.912 ± 0.009*
0.788 ± 0.008*
0.622 ± 0.006*
0.522 ± 0.006*
0.336 ± 0.005*
--
--
14.2
31.8
56.3
70.5
97.6
1.25

Hexane-ethanol extract CControl
HHeat killed
TPlant extract
--
--
0.50
1.00
2.00
5.00
10.0
24
0.5
24.0
20.0
15.0
10.0
5.0
1.008 ± 0.002
0.322 ± 0.004
0.955 ± 0.005*
0.87 ± 0.002*
0.745 ± 0.002*
0.56 ± 0.009*
0.345 ± 0.003*
--
--
7.8
20.7
38.4
65.4
96.7
3.6  

Ciprofloxacin CControl
HHeat killed
TAntibiotics  
--
--
1.0
2.0
5.0
10.0
20.0
24
0.5
24.0
20.0
15.0
10.0
5.0
1.011 ± 0.003
0.323 ± 0.001
0.965 ± 0.002*
0.886 ± 0.004*
0.688 ± 0.005*
0.551 ± 0.011*
0.342 ± 0.002*
--
--
6.7
18.2
47
66.9
97.3
7.5  

C Positive control, HNegative control, TTreated worm with extract/Ciprofloxacin
*P value represents the level of significance P<0.05 when comparing the mean value of absorbance observed for the formazan formed
between treated and control worms.

Singh et al.Trends in Vector Research and Parasitology  2014 1:1DOI : 10.7243/2054-9881-1-1