Pathology Discovery

Pathology Discovery

ISSN 2052-7896
Original Research

Neospora caninum as causative agent of ovine encephalitis in Iran

Farhang Sasani1*, Javad Javanbakht1, Parvaneh Seifori2, Saeid Fathi3 and Mehdi Aghamohammad Hassan4

*Correspondence: Farhang Sasani Fsasani@ut.ac.ir

1. Department of Pathology, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran.


Author Affiliations

2. Department of Pathology, Veterinary organization of Iran, Tehran, Iran.
3. Department of Parasitology, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran.
4. Department of Clinical Sciences, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran.

Abstract

Background: Neospora caninum, an apicomplexan parasite closely related to Toxoplasma gondii, causes abortion, stillbirths, and congenital neurologic disease in multiple animal species.The principle method of diagnosing N. caninum infection in aborted fetuses is by histopathology (HP) of fetal tissues. A sensitive and specific PCR detection assay for N. caninum DNA would be useful to augment the diagnosis of N. caninum abortion where pathologic changes in fetal tissues are consistent with neosporosis. The aim of this study was to investigate the presence of the encephalitis with focal and multifocal brain lesions that induced N.caninum abortion in sheep.

Methods: During 2004 to 2008, 109 aborted fetus's brains of sheep were collected to identify the possible causes of abortion from different provinces of Iran and were investigated in the pathology laboratory. Foetal histopathology was used to detect the presence of protozoal-associated lesions in brain. The presence of N. caninum was confirmed by Semi-nested PCR.

Results: Histopathological examination of this case demonstrated extensive suppurative and nonsuppurative meningoencephalitis, suppurative meningitis and non-septic and septic encephalitis. Along with various lesions incidence of cellular and vascular, the glial reactions were also assessed in aborted fetal brain tissues containing gliosis (focal or diffuse). During the process of extracting DNA from 109 selected samples, DNA extracted only in one case by semi-nested PCR test was positive for the presence of N.caninum.

Conclusions: This study demonstrates that N. caninum can be agent of brain lesions in sheep. This is the first report of N. caninum in sheep from Iran that indicated encephalitis brain lesion in hemispheres of the brain cortex.

Keywords: Neospora caninum, semi-nested PCR, histopathological, sheep, abortion

ISSN 2052-7896
Volume 1
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