Figure 3 : Tri-lineage differentiation of SF-CPs.

Third passage SF-CPs were maintained in basal medium (A, C and E) or were transferred to osteogenic (B, D), adipogenic (F) or chondrogenic (G, H) culture conditions. Osteogenic monolayers stained with Alizarin red (B) exhibited strong staining of multicellular aggregates that was absent in control cultures (A). The osteogenic aggregates also exhibited strong ALP activity, as demonstrated by intense purple staining in (D). Intracellular lipid accumulation, detected with Oil red O, was evident in SF-CPs maintained in adipogenic medium (F), but was absent in control cultures (E). In chondrogenic pellet cultures, SF-CPs synthesized a dense cartilaginous matrix that stained strongly for sulfated glycosaminoglycans with toluidine blue after 10 (G) and 20 days (H) in culture.

Stewart et al.Stem Cell Biology and Research  2016 3:1DOI : 10.7243/2054-717X-3-1