Table 5 : Adverse effects observed in the RACB study with caffeine with Sprague Dawley rats [26].


NOAEL
(mg/(kg bw x d))
LOAEL
(mg/(kg bw x d))
Sex/generation Endpointa Effect
(I: increased,
D: decreased
A: accelerated,
C: changed,
0: no effect)
Estrogen-mediated activity Androgen-mediated activity Thyroid-
related activity
Steroido-
genesis-related
activity
Endpoint potentially
sensitive to EATS modality
agonistic ant-agonistic agonistic ant-agonistic

-- 12.5 F + M/F0, F1 Body weight D -- -- -- -- -- -- --
50 > 50 M/F0, F1 Organ histology: testes, epididymis 0 -- -- -- -- -- -- --
-- 12.5 M/F0, F1 Sperm parameter:
percentage motile sperm, sperm velocity,
sperm average radius
D x x x x   x x
25 50 F0 Development: Live pup weight/litter D -- -- -- -- -- -- x
50 > 50 F0 Fertility, litter size, no. live pups, sex ratio 0 -- -- -- --   -- --
25 50 F1 Fertility: no. live pups/litter, proportion of
pups born alive
D -- -- -- -- -- -- x

a:significant changes relative to control

Schuhmacher-Wolz et al.Journal of Toxicology and Health  2017 4:2DOI : 10.7243/2056-3779-4-2