Figure 1 : IFN-γ secreting cells detected by ELISPOT in bovine PBMCs stimulated with H. lineatum antigens.

Cells from non-sensitized (□) and sensitized (■) cattle were incubated in absence (−PHA) and presence (+PHA) of the mitogen PHA. Results are presented as the average and standard deviation. Axis y: Score 0: no spots. Score 1:<50 spots/well. Score 2: 50-100 spots/well. Score 3: 100-200 spots/well. Score 4: 200-400 spots/well. Score 5: >400 spots/well.

Panadero et al.Veterinary Medicine and Animal Sciences  2016 4:2DOI : 10.7243/2054-3425-4-2