Veterinary Medicine and
Animal Sciences

Veterinary Medicine and Animal Sciences

ISSN 2054-3425
Original Research

Eicosapentaenoic acid and docosahexaenoic acid moderate inflammation in porcine cartilage explants

Cara I. Robison1 and Michael W. Orth2*

*Correspondence: Michael W. Orth

2. Department of Animal and Food Sciences, Texas Tech University, Box 42141, Lubbock, TX, USA.

Author Affiliations

1. Department of Animal Science, Michigan State University, 474 S. Shaw Lane, Anthony Hall Room 1290, East Lansing, MI, USA.


Objective: To characterize the effects of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) and arachidonic acid (ARA) in various combinations on indices of inflammation in recombinant porcine IL-1 beta stimulated porcine articular cartilage explants.

Methods: Cartilage was obtained from the humeral-ulnar joints of Yorkshire x Landrace market sized gilts. Explants were harvested from the humeral-ulnar joints within 8 h of slaughter. Explants were allocated to culture plates and cultured in 1 mL of Dulbecco's Modified Eagle serum free medium for 24 h with 10% fetal bovine serum. At 48 and 72 h, 1 mL of treatment media containing fatty acids and 15 ng/mL of recombinant porcine IL-1 was added to each well. At 48, 72, and 96 h after cartilage was allocated to wells, media were removed from each well and reserved for analysis. Media were analyzed for proteoglycan, nitric oxide (NO), interleukin-6 and prostaglandin E2concentrations.

Results: In general, when EPA and/or DHA are supplemented to explants in combination with linoleic acid (LA) NO and prostaglandin E2 release is decreased. Explants treated with 25 μg/mL DHA released 53% less NO into the media than explants treated with the same level of EPA and 60% less than explants treated with LA alone.

Conclusions: These data demonstrate that EPA and DHA are capable of modulating the inflammatory response on porcine articular cartilage in vitro.

Keywords: Cartilage, omega-3, omega-6, fatty acids, swine

ISSN 2054-3425
Volume 3
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