Figure 4 : Cell-free (A,B,C) and cell-associated (D,E,F) HCMV infection of the human coronary transfilter co-culture model:


(A) 14 days after cell-free HCMV infection, toluidin-blue staining, frontal cut through the filter membrane of the transfilter co-culture model, F = filter. (B) 14 days after cell-free HCMV infection, staining against HCMV early antigen, view from the top on the endothelial side of the filter membrane, F = filter. (C) 14 days after cell-free HCMV infection, positive staining against BrdU for the analysis of reactive cell proliferation and against von Willebrand factor for the identification of endothelial cells, frontal cut through the filter membrane, black arrow = BrdU-positive human coronary smooth muscle cells, F = filter. (D) 14 days after cell-associated HCMV infection, toluidin-blue staining, frontal cut through the filter membrane of the transfilter co-culture model, F = filter, bar = 40 μm. (E) 7 days after cell-associated HCMV infection, staining against CD68 for identification of monocytes, view from the top on the endothelial side of the filter membrane, white arrow. (F) 14 days after cell-associated HCMV infection, positive staining against BrdU (black arrow) for the analysis of reactive cell proliferation and against von Willebrand factor (red arrow) for the identification of endothelial cells.

Voisard et al.Virology Discovery  2013 1:2DOI : 10.7243/2052-6202-1-2