Table 1 : A brief outline of different genome-wide approaches aimed at identifying cellular factors associated with
influenza A virus replication.


Group Screening approach Cell line used Virus strain Host Gene/Factors
Targeted
No. of Hits

[42] Mass spectrometry Vero A/WSN/33 (H1N1) Host factors packaged
within virion particle
36
[40] Mass spectrometry HEK293T A/WSN/33 (H1N1) Extracts from infected
HEK293T cells
41
[43] RNAi Screen HEK293T, A549 Polymerases and vRNPs of
A/WSN/33 (H1N1);
A/VN/1203/04 (H5N1)
31 18
[32] RNAi Screen Drosophila cells Recombinant virus
A/WSN/33 (H1N1)
13,071 110
[34] RNAi Screen U2OS A/Puerto Rico/8/34 (H1N1) 17,877 133
[38] RNAi Screen A549 A/WSN/33 (H1N1) 22,843 227
[37] RNAi Screen A549 Recombinant virus
A/WSN/33 (H1N1)
19,628 295
[39] Yeast 2 Hybrid,
microarry, RNAi screen
HBEC A/Puerto Rico/8/34 (H1N1);
A/Udorn/307/72 (H3N2)
1,745 606
[41] RHGP MDCK A/Udorn/307/72 (H3N2) Host genes conferring
influenza resistance to
infected cells
110

Lal et al.Virology Discovery  2014 2:2DOI : 10.7243/2052-6202-2-2